Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 410051
Title Analysis of natural allelic variation in Arabidopsis using a multiparent recombinant inbred line population
Author(s) Huang, X.; Caldas Paulo, M.J.; Boer, M.P.; Effgen, S.; Keizer, P.; Koornneef, M.; Eeuwijk, F.A. van
Source Proceedings of the National Academy of Sciences of the United States of America 108 (2011)11. - ISSN 0027-8424 - p. 4488 - 4493.
DOI http://dx.doi.org/10.1073/pnas.1100465108
Department(s) Laboratory of Plant Breeding
Biometris (PPO/PRI)
Biometris (WU MAT)
Laboratory of Genetics
EPS
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) quantitative trait loci - flowering-time - complex traits - genetic architecture - collaborative cross - maize - power - qtl - thaliana - resolution
Abstract To exploit the diversity in Arabidopsis thaliana, eight founder accessions were crossed to produce six recombinant inbred line (RIL) subpopulations, together called an Arabidopsis multiparent RIL (AMPRIL) population. Founders were crossed pairwise to produce four F1 hybrids. These F1s were crossed according to a diallel scheme. The resulting offspring was then selfed for three generations. The F4 generation was genotyped with SNP and microsatellite markers. Data for flowering time and leaf morphology traits were determined in the F5 generation. Quantitative trait locus (QTL) analysis for these traits was performed using especially developed mixed-model methodology, allowing tests for QTL main effects, QTL by background interactions, and QTL by QTL interactions. Because RILs were genotyped in the F4 generation and phenotyped in the F5 generation, residual heterozygosity could be used to confirm and fine-map a number of the QTLs in the selfed progeny of lines containing such heterozygosity. The AMPRIL population is an attractive resource for the study of complex traits.
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