Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 410282
Title Chemoenzymatic synthesis of biotin-appended analogues of gangliosides GM2, GM1, GD1 and GalNAc-GD1a for solid-phase applications and improved ELISA tests
Author(s) Pukin, A.; Florack, D.E.A.; Brochu, D.; Lagen, B. van; Visser, G.M.; Wennekes, T.; Gilbert, M.; Zuilhof, H.
Source Organic & Biomolecular Chemistry 9 (2011)16. - ISSN 1477-0520 - p. 5809 - 5815.
DOI https://doi.org/10.1039/c1ob00009h
Department(s) Laboratory for Organic Chemistry
PRI Bioscience
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) surface-plasmon resonance - carbohydrate microarrays - campylobacter-jejuni - cholera-toxin - oligosaccharide microarrays - biological-systems - guillain-barre - nmr analysis - binding - sugar
Abstract Biotinylated analogues of gangliosides GM2, GM1, GD1a and GalNAc-GD1a were synthesized in high yields using glycosyltransferases from Campylobacter jejuni. The presence of a biotin moiety in the aglycone part of these mimics allows for attachment of these materials onto various streptavidin-coated surfaces. Analysis of the interaction of biotin-appended GM1 with the B subunit of Escherichia coli heat-labile enterotoxin performed in a modified ELISA procedure shows the potential of this compound to replace the natural GM1 in toxin detection.
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