Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 410369
Title The transcription machinery underlying flower formation
Author(s) Kaufmann, K.; Smaczniak, C.D.; Angenent, G.C.
Source NPC Highlights 2009 (2009)9. - p. 5 - 7.
Department(s) PRI Bioscience
Laboratory of Molecular Biology
PRI BIOS Plant Development Systems
Publication type Article in professional journal
Publication year 2009
Abstract The Kluyver Centre for Genomics of Industrial Fermentations and the Netherlands Proteomics Centre collaborate in a Hotel Project to study secretion of proteins in the mycelium of the filamentous fungus Aspergillus niger. Han Wösten’s Microbiology group and Albert Heck’s Biomolecular Mass Spectrometry and Proteomics group analyze the secretome of this filamentous fungus that is used as a cell factory for industrial proteins. In this study, state of the art proteomics and genomics will be combined to obtain more insight in which parts of the colony actually contribute to secretion of (sets of) proteins. By using a defined culturing technique, secretion of enzymes at different zones of the fungus can be harvested. Using an advanced mass spectrometric technique, about seventy enzymes were identified at the periphery of the fungus. Of the proteins identified, all but one showed a predicted signal sequence for secretion. This shows that lysis of cells is minor, if present at all, in this zone of the colony. Lysis seems also absent in the colony centre, despite the fact that this part of the colony was already seven days old. Remarkably, six proteins that were not produced at the periphery of the colony were secreted in this zone. Thus, heterogeneity is reflected in the secretion of the mycelium. With this knowledge production of industrial relevant proteins can be improved
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