Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 410482
Title A DNA-based strategy for dynamic positional enzyme immobilization inside fused silica microchannels
Author(s) Vong, T.H.; Schoffelen, S.; Dongen, S.F.M. van; Beek, T.A. van; Zuilhof, H.; Hest, J.C.M. van
Source Chemical Science 2 (2011)7. - ISSN 2041-6520 - p. 1278 - 1285.
Department(s) Laboratory for Organic Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) directed immobilization - microarrays - proteins - reactors - monoliths
Abstract A three-enzyme cascade reaction was successfully realized in a continuous flow microreactor. The first enzyme (Candida antarctica lipase B, also known as Pseudozyma antarctica lipase B) and the third enzyme (horseradish peroxidase) of the cascade process were immobilized in a mild non-contact manner via ssDNA-ssDNA interaction in discrete zones on the capillary wall, whereas the second enzyme (glucose oxidase) was kept in the mobile phase. The unique combined feature of patterning, possibility of loading and stripping, and modularity in a fused silica microchannel is demonstrated. By changing the distance between the two enzyme patches, the reaction time available for glucose oxidase could be independently and modularly varied. The reusability of the enzymatic microfluidic system was shown by using the hybridization and dehybridization capabilities of DNA as a tool for subsequent enzyme immobilization and removal
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