Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 411454
Title Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens
Author(s) Overbeek, L.S. van; Smalla, K.; Bailey, M.; Jansson, J.; Sjöling, S.; Vogel, T.; Nalin, R.; Elsas, J.D. van
Source Wageningen : Plant Research International
Department(s) Biointeracties and Plant Health
Publication type Scientific report
Publication year 2008
Abstract Objective: This project will explore & exploit the anti-phytopathogen diversity present in selected European suppressive soils representing a wide geographical spread. The untapped functional potential present in the unculturable part of the microbiota of these soils will be accessed using metagenomic cloning of soil DNA. Moreover, as a baseline, antagonistic organisms will also be isolated. Libraries constructed on the basis of the total and the active cell fractions, as well as the isolates, will be screened for anti-phytopathogen function. Selected clones will be as said with respect to the nature of the antagonistic compounds and production loci, involving sequencing. Potential producer strains will be optimised as to gene expression, growth and stability. Based on the gene sequences obtained, molecular tools will be developed and used to monitor soil suppressiveness. The impact (biosafety) of potential applications will be assessed.
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