Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 411530
Title Fast chromatographic separation for the quantitation of the main flavone dyes in Reseda luteola (weld)
Author(s) Villela, A.; Klift, E.J.C. van der; Mattheussens, E.S.G.M.; Derksen, G.C.H.; Zuilhof, H.; Beek, T.A. van
Source Journal of Chromatography. A, Including electrophoresis and other separation methods 1218 (2011)47. - ISSN 0021-9673 - p. 8544 - 8550.
DOI http://dx.doi.org/10.1016/j.chroma.2011.09.069
Department(s) Laboratory for Organic Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) performance liquid-chromatography - diode-array detection - l. - identification - quantification - bilobalide - portugal
Abstract In the past decades, there has been a renewed interest in the use of natural dye plants for textile dyeing, e.g. Reseda luteola (weld). Its main yellow dye constituents are the flavones luteolin-7,3'-O-diglucoside, luteolin-7-O-glucoside and luteolin. The aim of this work was to develop a simple validated industrially usable quantitative method to assess the flavone content of R. luteola samples. The flavones were overnight extracted from the dried and ground aerial parts of the plant at room temperature via maceration with methanol-water 8:2. Afterwards, they were quantified through internal standardisation against chrysin by RP-HPLC-UV at 345nm. The efficiency of the one-step extraction was 95%. The limits of detection (LOD) and quantitation (LOQ) were =1ng and =3ng, respectively, providing ample sensitivity for the purpose. The precision expressed as relative standard deviation of the entire method was
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