Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 411958
Title Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge
Author(s) Luesken, F.; Alen, T. van; Biezen, J. van der; Frijters, C.; Toonen, G.; Kampman, C.; Hendrickx, T.L.G.; Zeeman, G.; Temmink, B.G.; Strous, M.; Camp, H.J.M. op den; Jetten, M.S.M.
Source Applied Microbiology and Biotechnology (2011). - ISSN 0175-7598
Department(s) Sub-department of Environmental Technology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) afvalwaterbehandeling - waterzuivering - anaërobe behandeling - methaan - oxidatie - bacteriën - nieuwe stam - denitrificerende micro-organismen - waste water treatment - water treatment - anaerobic treatment - methane - oxidation - bacteria - new phylum - denitrifying microorganisms - activated-sludge - microorganisms - populations - technology - reactor
Categories Waste Water Treatment
Abstract Recently discovered microorganisms affiliated to the bacterial phylum NC10, named “Candidatus Methylomirabilis oxyfera”, perform nitrite-dependent anaerobic methane oxidation. These microorganisms could be important players in a novel way of anaerobic wastewater treatment where ammonium and residual dissolved methane might be removed at the expense of nitrate or nitrite. To find suitable inocula for reactor startup, ten selected wastewater treatment plants (WWTPs) located in The Netherlands were screened for the endogenous presence of M. oxyfera using molecular diagnostic methods. We could identify NC10 bacteria with 98% similarity to M. oxyfera in nine out of ten WWTPs tested. Sludge from one selected WWTP was used to start a new enrichment culture of NC10 bacteria. This enrichment was monitored using specific pmoA primers and M. oxyfera cells were visualized with fluorescence oligonucleotide probes. After 112 days, the enrichment consumed up to 0.4 mM NO2- per day. The results of this study show that appropriate sources of biomass, enrichment strategies, and diagnostic tools existed to start and monitor pilot scale tests for the implementation of nitrite-dependent methane oxidation in wastewater treatment at ambient temperature
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