Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 411970
Title Strigolactone Biosynthesis in Medicago truncatula and Rice Requires the Symbiotic GRAS-Type Transcription Factors NSP1 and NSP2
Author(s) Liu, W.; Kohlen, W.; Lillo, A.; Camp, R. op den; Ivanov, S.; Hartog, M.; Limpens, E.H.M.; Jamil, M.; Smaczniak, C.; Kaufmann, K.; Yang, W.C.; Hooiveld, G.J.E.J.; Charnikhova, T.; Bouwmeester, H.J.; Bisseling, T.; Geurts, R.
Source The Plant Cell 23 (2011)10. - ISSN 1040-4651 - p. 3853 - 3865.
DOI http://dx.doi.org/10.1105/tpc.111.089771
Department(s) EPS-1
VLAG
Laboratory of Molecular Biology
Laboratory of Plant Physiology
PRI Bioscience
Chair Nutrition Metabolism and Genomics
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) arbuscular mycorrhizal fungi - affymetrix genechip data - tiller bud outgrowth - quality assessment - lotus-japonicus - germination stimulants - transduction pathway - phosphate deficiency - parasitic plants - red-clover
Abstract Legume GRAS (GAI, RGA, SCR)-type transcription factors NODULATION SIGNALING PATHWAY1 (NSP1) and NSP2 are essential for rhizobium Nod factor-induced nodulation. Both proteins are considered to be Nod factor response factors regulating gene expression after symbiotic signaling. However, legume NSP1 and NSP2 can be functionally replaced by nonlegume orthologs, including rice (Oryza sativa) NSP1 and NSP2, indicating that both proteins are functionally conserved in higher plants. Here, we show that NSP1 and NSP2 are indispensable for strigolactone (SL) biosynthesis in the legume Medicago truncatula and in rice. Mutant nsp1 plants do not produce SLs, whereas in M. truncatula, NSP2 is essential for conversion of orobanchol into didehydro-orobanchol, which is the main SL produced by this species. The disturbed SL biosynthesis in nsp1 nsp2 mutant backgrounds correlates with reduced expression of DWARF27, a gene essential for SL biosynthesis. Rice and M. truncatula represent distinct phylogenetic lineages that split approximately 150 million years ago. Therefore, we conclude that regulation of SL biosynthesis by NSP1 and NSP2 is an ancestral function conserved in higher plants. NSP1 and NSP2 are single-copy genes in legumes, which implies that both proteins fulfill dual regulatory functions to control downstream targets after rhizobium-induced signaling as well as SL biosynthesis in nonsymbiotic conditions.
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