Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 411985
Title Pea powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus
Author(s) Pavan, S.N.C.; Schiavulli, A.; Appiano, M.; Visser, R.G.F.; Bai, Y.
Source Theoretical and Applied Genetics 123 (2011)8. - ISSN 0040-5752 - p. 1425 - 1431.
DOI http://dx.doi.org/10.1007/s00122-011-1677-6
Department(s) Laboratory of Plant Breeding
WUR Plant Breeding
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) pisum-sativum l. - gene - markers - er-1 - ol-2 - rapd
Abstract The powdery mildew disease affects several crop species and is also one of the major threats for pea (Pisum sativum L.) cultivation all over the world. The recessive gene er1, first described over 60 years ago, is well known in pea breeding, as it still maintains its efficiency as a powdery mildew resistance source. Genetic and phytopathological features of er1 resistance are similar to those of barley, Arabidopsis, and tomato mlo powdery mildew resistance, which is caused by the loss of function of specific members of the MLO gene family. Here, we describe the obtainment of a novel er1 resistant line by experimental mutagenesis with the alkylating agent diethyl sulfate. This line was found to carry a single nucleotide polymorphism in the PsMLO1 gene sequence, predicted to result in premature termination of translation and a non-functional protein. A cleaved amplified polymorphic sequence (CAPS) marker was developed on the mutation site and shown to be fully co-segregating with resistance in F2 individuals. Sequencing of PsMLO1 from three powdery mildew resistant cultivars also revealed the presence of loss-of-function mutations. Taken together, results reported in this study strongly indicate the identity between er1 and mlo resistances and are expected to be of great breeding importance for the development of resistant cultivars via marker-assisted selection.
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