Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 412050
Title Further characterization of tulip severe mosaic virus supports classification as a member of genus Ampellovirus in the family Closteroviridae
Author(s) Pham, K.T.K.; Blom-Barnhoorn, G.J.; Bijman, V.P.; Lemmers, M.E.C.; Derks, A.F.L.M.
Source In: Proceedings of the XII International Symposium on Virus Diseases of Ornamental Plants. - Haarlem : ISHS - ISBN 9789066052536 - p. 239 - 246.
Event Haarlem : ISHS - ISBN 9789066052536 XII International Symposium on Virus diseases of ornamental plants, Haarlem, 2008-04-20/2008-04-24
Department(s) Flower Bulbs
Nursery Stock-Flower Bulbs
Publication type Contribution in proceedings
Publication year 2011
Abstract Tulip bulb and flower production greatly contribute to the economy in The Netherlands. However, severe loss in this industry is caused by different viruses. In recent years, diagnostic tools were developed for most of these viruses with Tulip severe mosaic virus (TSMV) as one of the few exceptions. To further characterize TSMV, the virus was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with degenerate primers targeted to conserved sequences of the Heat shock protein 70 gene of viruses from the family Closteroviridae. Phylogenetic analysis of the 0.5 kb HSP70 gene fragments showed that TSMV is closely related to Plum bark necrosis stem pitting-associated virus (PBNSPaV), Apricot stem pitting-associated virus (ASPaV) and Pineapple mealybug wilt-associated virus 1 (PMWaV-1), all three members of the genus Ampellovirus. In addition, a set of primers amplifying a TSMV-specific fragment was designed that enables fast and specific detection of the virus. Additionally to the molecular experiments, two possible modes of virus transmission were tested, viz. by aphids and by soil-borne organisms. However, no transmission was recorded by both methods.
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