Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 412424
Title A fast ethanol assay to detect seed deterioration
Author(s) Kodde, J.; Buckley, W.T.; Groot, C.C. de; Retiere, M.; Víquez Zamora, A.M.; Groot, S.P.C.
Source Seed Science Research 22 (2012). - ISSN 0960-2585 - p. 55 - 62.
Department(s) PRI BIOS Plant Development Systems
WUR Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) physical sanitation treatments - maize zea-mays - cabbage seeds - germination - acetaldehyde - fermentation - mitochondria - sensitivity - performance - metabolism
Abstract The most common way to test seed quality is to use a simple and reliable but time- and space-consuming germination test. In this paper we present a fast and simple method to analyse cabbage seed deterioration by measuring ethanol production from partially imbibed seeds. The method uses a modified breath analyser and is simple compared to gas chromatographic or enzymatic procedures. A modified method using elevated temperatures (40°C instead of 20°C) shortened the assay time and improved its sensitivity. The analysis showed an inverse correlation between ethanol production and seed quality (e.g. the final percentages or speed of germination and the number of normal seedlings). The increase in ethanol production was observed when cabbage seeds were deteriorated by storage under ambient conditions or hot water treatments, both of which reduced the number of normal seedlings. Premature seeds produced more ethanol upon imbibition than mature seeds. Ethanol production occurred simultaneously with oxygen consumption, indicating that lack of oxygen is not the major trigger for ethanol production.
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