Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 412437
Title Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp.
Author(s) Schwenzfeier, A.; Wierenga, P.A.; Gruppen, H.
Source Bioresource Technology 102 (2011)19. - ISSN 0960-8524 - p. 9121 - 9127.
DOI https://doi.org/10.1016/j.biortech.2011.07.046
Department(s) Food Chemistry Group
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) expanded-bed adsorption - marine microalgae - chromatography - chlorella - hydrolysis - glycinin - nitrogen - biomass - cells - acid
Abstract Extraction of high-value protein fractions for techno-functional applications in foods can considerably increase the commercial value of microalgae biomass. Proteins from Tetraselmis sp. were extracted and purified after cell disintegration by bead milling, centrifugation, ion exchange chromatography using the absorbent Streamline DEAE, and final decolorization by precipitation at pH 3.5. The algae soluble isolate was free from the intense color typical for algae products and contained 64% (w/w) proteins and 24% (w/w) carbohydrates. The final isolate showed solubility independent of ionic strength and 100% solubility at and above pH 5.5. Since most plant proteins used in foods show poor solubility in the pH range 5.5–6.5, the algae soluble protein isolate could be useful for techno-functional applications in this pH range.
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