Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 412846
Title The Plastic Finished Genome of the Fungal Wheat Pathogen Mycosphaerella graminicola
Author(s) Kema, G.H.J.; Goodwin, S.B.; M'Barek, S. Ben; Wittenberg, A.H.J.
Source In: Proceedings of the Fourth Annual DOE Joint Genome Institute User Meeting on Genomics of Energy & Environment, Walnut Creek, California, USA, 25-27 March 2009. - U.S. Department of Energy, Office of Science - p. 31 - 31.
Event Fourth Annual DOE Joint Genome Institute User Meeting on Genomics of Energy & Environment, Walnut Creek, California, USA, 2009-03-25/2009-03-27
Department(s) PRI BIOINT Moleculair Phytopathology
Laboratory of Phytopathology
Biointeracties and Plant Health
PRI Biodiversity and Breeding
Publication type Abstract in scientific journal or proceedings
Publication year 2009
Abstract Meiosis in the haploid plant-pathogenic fungus Mycosphaerella graminicola results in eight ascospores due to a mitotic division following the two meiotic divisions. The transient diploid phase allows for recombination among homologous chromosomes. However, some chromosomes of M. graminicola lack homologs and do not pair during meiosis. Because these chromosomes are not present universally in the genome of the organism they can be considered to be dispensable. Detailed genetic analyses of two high density mapping populations revealed that M. graminicola has 21 chromosomes including up to eight dispensable chromosomes, the highest number reported in filamentous fungi. These chromosomes vary from 0.41 to 0.77 Mb in size, representing 38% of the chromosome number and 11.6% of the genome. Chromosome numbers among progeny isolates varied widely, with some progeny missing up to three chromosomes, while other strains were disomic for one or more chromosomes. Between 15- 20% of the progeny isolates lacked one or more chromosomes that were present in both parents. The two high-density maps showed no recombination of dispensable chromosomes and hence, their meiotic processing may require distributive disjunction, a phenomenon that is rarely observed in fungi. The maps also enabled the identification of individual twin isolates from a single ascus that shared the same missing or doubled chromosomes indicating that the chromosomal polymorphisms were mitotically stable and originated from nondisjunction during the second division and, less frequently, during the first division of fungal meiosis. High genome plasticity could be among the strategies enabling this versatile pathogen to quickly overcome adverse biotic and abiotic conditions in wheat fields. Additionally, we used a Comparative Genomic Hybridization whole-genome array based on the finished genome of M. graminicola ( This confirmed that chromosomes 14-21 were frequently absent among isolates, without visible effect on viability or virulence, whereas chromosomes 1-13 were invariably present. The dispensable chromosomes are smaller and have significantly lower gene densities. Most of their genes are duplicated on the essential chromosomes and show a different codon usage. Dispensable chromosomes also contained a higher density of transposons, pseudogenes, and unclassified genes, which could encode novel proteins. Moreover, the dispensable chromosomes show extremely low synteny with other Dothideomycete genomes
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.