Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 412964
Title Comparison of anonymous and targeted molecular markers for the estimation of genetic diversity in ex situ conserved Lactuca
Author(s) Treuren, R. van; Hintum, T.J.L. van
Source Theoretical and Applied Genetics 119 (2009)7. - ISSN 0040-5752 - p. 1265 - 1279.
DOI https://doi.org/10.1007/s00122-009-1131-1
Department(s) Centre for Genetic Resources, The Netherlands
Publication type Refereed Article in a scientific journal
Publication year 2009
Keyword(s) sativa l. - lettuce - aflp - polymorphism - genome - populations - collection - resistance - ssap - srap
Abstract The anonymous marker systems microsatellites (simple sequence repeats), amplified fragment length polymorphisms and sequence-specific amplified polymorphisms were compared with the targeted marker systems sequence-related amplified polymorphisms, target region amplification polymorphisms and nucleotide binding site profiling for their ability to describe the genetic diversity in a selected set of 80 Lactuca accessions. The accessions were also described morphologically, and all characterisation methods were evaluated against the genetic diversity assessed by a panel of three crop experts. The morphological data showed a low level of association with the molecular data, and did not display a consistently better relationship with the experts’ assessments in comparison with the molecular data. In general, the diversity described by the targeted molecular markers did not differ markedly from that of the anonymous markers, resulting in only slight differences in performance when related to the expert-based assessments. It was argued that markers targeted to specific gene sequences may still behave as anonymous markers and that the type of marker system used is irrelevant when at low taxonomic levels a clear genetic structure is absent due to intensive breeding activities
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