Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 413519
Title Millifluidic droplet analyser for microbiology
Author(s) Baraban, L.; Bertholle, F.; Salverda, M.L.M.; Bremond, N.; Panizza, P.; Baudry, J.; Visser, J.A.G.M. de; Bibette, J.
Source Lab on a Chip 11 (2011). - ISSN 1473-0197 - p. 4057 - 4062.
DOI https://doi.org/10.1039/c1lc20545e
Department(s) Laboratory of Genetics
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) stochastic gene-expression - escherichia-coli - single cells - bacteria - susceptibility - resistance - microfluidics - encapsulation - consequences - antibiotics
Abstract We present a novel millifluidic droplet analyser (MDA) for precisely monitoring the dynamics of microbial populations over multiple generations in numerous (=103) aqueous emulsion droplets (100 nL). As a first application, we measure the growth rate of a bacterial strain and determine the minimal inhibitory concentration (MIC) for the antibiotic cefotaxime by incubating bacteria in a fine gradient of antibiotic concentrations. The detection of cell activity is based on the automated detection of an epifluorescent signal that allows the monitoring of microbial populations up to a size of 106 cells. We believe that this device is helpful for the study of population dynamic consequences of microbe-environment interactions and of individual cell differences. Moreover, the fluidic machine may improve clinical tests, as it simplifies, automates and miniaturizes the screening of numerous microbial populations that grow and evolve in compartments with a finely tuned composition
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