Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 413529
Title EBR1, a novel Zn2Cys6 transcription factor, affects virulence and apical dominance of hyphal tip in Fusarium graminearum
Author(s) Zhao, C.; Waalwijk, C.; Wit, P.J.G.M. de; Lee, T.A.J. van der; Tang, D.
Source Molecular Plant-Microbe Interactions 24 (2011)12. - ISSN 0894-0282 - p. 1407 - 1418.
DOI http://dx.doi.org/10.1094/MPMI-06-11-0158
Department(s) Laboratory of Phytopathology
PRI BIOINT Moleculair Phytopathology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) multiple sequence alignment - aspergillus-nidulans - gibberella-zeae - saccharomyces-cerevisiae - aflatoxin biosynthesis - binuclear cluster - neurospora-crassa - reduced virulence - female fertility - plant infection
Abstract Zn2Cys6 transcription factors are unique to fungi and have been reported to be involved in different regulatory functions. Here, we characterized EBR1 (enhanced branching 1), a novel Zn2Cys6 transcription factor of Fusarium graminearum. Knocking out EBR1 in F. graminearum PH-1 caused reduction of both radial growth and virulence. The conidia of knock-out strain PH-1¿ebr1 germinated faster than those of wild-type PH-1, but the conidiation of the mutant was significantly reduced. Detailed analysis showed that the reduced radial growth might be due to reduced apical dominance of the hyphal tip, leading to increased hyphal branching. Inoculation assays on wheat heads with a green fluorescent protein (GFP)-labeled PH-1¿ebr1 mutant showed that it was unable to penetrate the rachis of the spikelets. Protein fusion with GFP showed that EBR1 is localized in the nucleus of both conidia and hyphae. Knocking out the orthologous gene FOXG_05408 in F. oxysporum f. sp. lycopersici caused a much weaker phenotype than the PH-1¿ebr1 mutant, which may be due to the presence of multiple orthologous genes in this fungus. Transformation of FOXG_05408 into PH-1¿ebr1 restored the mutant phenotype. Similar to EBR1, FOXG_05408 is localized in the nucleus of F. oxysporum f. sp. lycopersici. Possible functions of EBR1 and its relation with other fungal transcription factors are discussed
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