Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 414533
Title Comparative genome hybridization demonstrates loss of chromosomes in Mycosphaerella graminicola isolates
Author(s) M'Barek, S. Ben; Lee, T.A.J. van der; Wittenberg, A.H.J.; Schouten, H.J.; Souza, M.T.; Kema, G.H.J.
Source In: Proceedings of the 9th European Conference on Fungal Genetics, Edinburgh, Scotland, 5-8 April 2008. - ECFG - p. 70 - 70.
Event 9th European Conference on Fungal Genetics, Edinburgh, Scotland, 2008-04-05/2008-04-08
Department(s) Biointeracties and Plant Health
PRI BIOINT Moleculair Phytopathology
PRI Biodiversity and Breeding
WUR Plant Breeding
Publication type Abstract in scientific journal or proceedings
Publication year 2008
Abstract PR1.46: Comparative Genomic Hybridization (CGH) arrays using a high density whole genome tilling NimbleGen platform ( were employed to further understand the genome plasticity of Mycosphaerella graminicola, the causal agent of septoria tritici blotch of wheat. The availability of the finished genome of M. graminicola IPO323 (, detailed genetic maps and karyotypes enables genome wide analysis for variation in DNA copy number and provides a new window to study chromosome polymorphism and translocations. In total, 387000 probes based on the IPO323 genome, were spotted on the array. These microarrays consist of 50 to 75–mer probes with a mean probe spacing of 118bp and a maximum stringency of 2 covering therefore the whole genome. We have analyzed the parents and progeny isolates of the M. graminicola mapping population IPO94269 and the sequenced isolate IPO323. Hybridization of M. graminicola IPO94269 displayed substantial aneuploidy against the IPO323 reference genome. Our results reveal widespread deletions between these two parental isolates and confirm the absence of two complete chromosomes in isolate IPO94269 previously demonstrated by karyotyping (Mehrabi et al. 2007) and genetic linkage analysis. Our data indicate CGH arrays can be used to detect aneuploidy, to predict the sites of chromosome breaks, and to identify recombination breakpoints. We currently exploit CGH to identify large genome differences such as deletions or translocations between isolates to understand genome plasticity and its relation with virulence, host specificity and speciation.
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