Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 414534
Title Tagging Pathogenicity genes in Fusarium graminearum using the transposon system mimp/impala (Session 4: Pathogenesis and Plant Pathology)
Author(s) Dufresne, M.; Lee, T.A.J. van der; M'Barek, S. Ben; Xu, X.; Zhang, X.; Liu, T.; Kema, G.H.J.; Daboussi, M.J.; Waalwijk, C.
Source In: Cereal Research Communications. 3rd International Symposium on Fusarium Head Blight, Szeged, Hungary, 2-7 September 2008. - Budapest, Hungary : Akadémiai Kiadó - p. 415 - 419.
Event Budapest, Hungary : Akadémiai Kiadó 3rd International Symposium on Fusarium Head Blight, Szeged, Hungary, 2008-09-02/2008-09-07
Department(s) PRI BIOINT Moleculair Phytopathology
Biointeracties and Plant Health
Publication type Contribution in proceedings
Publication year 2008
Abstract Transposon mutagenesis was applied to generate mutants in Fusarium graminearum. The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon and reinserted at high frequency in (the vicinity) of genes. A collection of mutants was screened for growth, for pathogenicity and for perithecia production.Several mutants blocked in one or more functions were obtained. The wild-type phenotype of one such mutant could be restored by complementation with a non-disrupted copy of the gene. In addition reinsertions occurred on each of the four chromosomes of F. graminearum, making this system a powerful tool in the functional analyses of the > 10,000 genes predicted in the F. graminearum genome
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