Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 414536
Title Tagging pathogenicity genes in Fusarium graminearum using the transposon system Mimp/Impala
Author(s) Dufresne, M.; Lee, T.A.J. van der; M'Barek, S. Ben; Liu, T.; Waalwijk, C.; Zhang, W.; Kema, G.H.J.; Daboussi, M.J.
Source In: XXIV Fungal Genetics Conference, Asilomar, California, USA, 20-25 March 2007. - Kansas City, USA : FGSC - p. 166 - 166.
Event Kansas City, USA : FGSC XXIV Fungal Genetics Conference, Asilomar, USA, 2007-03-20/2007-03-25
Department(s) Biointeracties and Plant Health
Publication type Abstract in scientific journal or proceedings
Publication year 2007
Abstract The number of predicted genes present in the genome of Fusarium graminearum is estimated to be around 14,000. For many genes the function is yet unknown and consequently there is a need for a high-throughput method for functional analyses of genes. We applied a transposon mutagenesis strategy using a mite element (mimp1) activated by a transposase (impala). Previously we have shown that the double component system mimp1/impala transposase is fully functional in F. graminearum (Dufresne et al., 2006). Transposition characteristics and high reinsertion frequency were found to be the same as in the original host species, F. oxysporum, allowing the application of this double component system for the generation of large transposon mutant collections. We transformed the double component system into F. graminearum strain FG820, selected 100 revertants and determined the sequence flanking the mimp1 reinsertion sites using TAIL-PCR and the F. graminearum genome sequence. In 53% of the isolates mimp1 reinserted close to or in genes. Subsequently, a pilot collection of around 300 revertants from the same transformant (FG820-6-11) was screened for growth on a large set of media and for pathogenicity on wheat. Several revertants with altered phenotypes were identified and in one of them mimp1 reinserted into an ORF encoding a transcription factor (FG820-6-11-r112). The relationship between mimp1 insertion and the mutant phenotype is currently investigated by functional complementation. Our results indicate that this novel double component transposon system is a powerful mutagenesis tool for high-throughput analysis of F. graminearum and potentially other ascomycete fungi
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