Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 414953
Title Multiplex Screening of Persistent Organic Pollutants in Fish Using Spectrally Encoded Microspheres
Author(s) Meimaridou, A.; Kalachova, K.; Shelver, W.L.; Franek, M.; Pulkrabova, J.; Haasnoot, W.; Nielen, M.W.F.
Source Analytical Chemistry 83 (2011)22. - ISSN 0003-2700 - p. 8696 - 8702.
Department(s) RIKILT - R&C Diergeneesmiddelen
Laboratory for Organic Chemistry
RIKILT - R&C Groeibevorderaars
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) polybrominated diphenyl ethers - dioxin-like compounds - polycyclic aromatic-hydrocarbons - polychlorinated-biphenyls pcbs - magnetic particle immunoassay - health implications - mass-spectrometry - food samples - contamination - congeners
Abstract Persistent organic pollutants (POPs) are environmental and food-related contaminants of global public health concern and known to be carcinogenic and endocrine disruptors. Their monitoring is essential, and an easy-to-use, rapid, and affordable multianalyte screening method with simplified sample preparation can be a valuable tool prior to instrumental analysis. For this purpose, a flow cytometric immunoassay (FCIA), based on a spectrally encoded microbeads technology, was developed for the multiplex detection of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (BDEs) in buffer and fish extracts. The sensitivities of the assays in the three-plex FCIA format were similar to the individual FCIAs for the marker compounds benzo[a]pyrene (BaP), 3,3',4,4'-tetrachlorobiphenyl (PCB77), and 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in buffer with IC50 values of 0.4, 20, and 2 µg L–1, respectively. Apart from the three markers, we could detect at least 14 other POPs. Extracts of fish with different fat content, prepared with a simplified extraction and cleanup procedure, had an insignificant influence on the overall three-plex FCIA performance, with the exception of some impact on the PAHs detection. The performance of the three-plex FCIA, in combination with the simple extraction procedure, is adequate for regulatory control in accordance with the required limits.
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