Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 415041
Title A Detection Method for Tropical Race 4 of the Banana Pathogen Fusarium oxysporum f. sp. cubense
Author(s) Waalwijk, C.; Dita Rodriguez, M.A.; Buddenhagen, I.; Paiva, L.V.; Souza, M.T.; Kema, G.H.J.
Source In: Proceedings of the International ISHS-ProMusa Symposium on Global Perspectives on Asian Challenges, Guangzhou, China, 14-18 September 2009. - Leuven, Belgium : ISHS - ISBN 9789066051386 - p. 339 - 352.
Event Leuven, Belgium : ISHS - ISBN 9789066051386 The International ISHS-ProMusa Symposium on Global Perspectives on Asian Challenges, Guangzhou, China, 2009-09-14/2009-09-18
DOI http://dx.doi.org/10.17660/ActaHortic.2011.897.46
Department(s) PRI BIOINT Moleculair Phytopathology
PPO/PRI Biointeractions and Plant Health
Biointeracties and Plant Health
Publication type Contribution in proceedings
Publication year 2011
Abstract Fusarium oxysporum f. sp. cubense (Foc) is the causal agent of Fusarium wilt, the devastating disease that ruined the ‘Gros Michel’ (AAA)-based banana production in the first half of the 20th century. The occurrence of a new variant in Southeast Asia that overcomes the resistance in Cavendish clones such as ‘Grand Naine’ (AAA) is a major concern to current banana production worldwide. The threat posed by this new variant, called tropical race 4 (TR4), may be overcome by the introduction of resistant cultivars. However, the identification of new resistant sources or breeding for resistance is a long-term effort. Currently, the only option to control the disease is to avoid or reduce the spread of the pathogen by eradication of infected plants and isolation of infested plantations. This requires sensitive and highly specific diagnostics that enable early detection of the pathogen. A two-locus database of DNA sequences, from over 800 different isolates from multiple formae speciales of F. oxysporum, was used to develop a molecular diagnostic tool that specifically detects isolates from the vegetative compatibility group (VCG) 01213, which encompasses the Foc TR4 genotype. This diagnostic tool was able to detect all Foc TR4 isolates tested, while none of the Foc isolates from 19 VCGs other than 01213 showed any reaction. In addition, the developed diagnostic tool was able to detect Foc TR4 when using DNA samples from different tissues of ‘Grand Naine’ plants inoculated with TR4 isolates
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