Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 415044
Title Mechanism and control of Genipa americana seed germination
Author(s) Queiroz, S.E.E.; Silva, E.A.A. da; Davide, A.C.; Jose, A.C.; Silva, A.T.; Fraiz, A.C.R.; Faria, J.M.R.; Hilhorst, H.W.M.
Source Physiologia Plantarum 144 (2012)3. - ISSN 0031-9317 - p. 263 - 276.
DOI http://dx.doi.org/10.1111/j.1399-3054.2011.01554.x
Department(s) Laboratory of Plant Physiology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) endo-beta-mannanase - tomato lycopersicon-esculentum - endosperm cap - abscisic-acid - cell-wall - micropylar endosperm - radicle emergence - molecular-cloning - embryo - gene
Abstract Genipa americana (Rubiaceae) is important for restoration of riparian forest in the Brazilian Cerrado. The objective was to characterize the mechanism and control of germination of G. americana to support uniform seedling production. Morphology and morphometrics of seeds, embryo and endosperm were assessed by light and scanning electron microscopy during germination. Imbibition and germination curves were generated and over the same time interval endosperm digestion and resistance were measured by puncture force analysis and activity assay of endo-ß-mannanase in water and in abscisic acid. The gene encoding for endo-ß-mannanase was partially cloned and its expression monitored by qRT-PCR. Embryos displayed growth prior to radicle protrusion. A two-phase increase in endo-ß-mannanase activity coincided with the two stages of weakening of the micropylar endosperm. The second stage also coincided with growth of the embryo prior to radicle protrusion. Enzyme activity was initiated in the micropylar endosperm but spread to the lateral endosperm. ABA
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