Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 415301
Title Clostridium difficile in Dutch animals: their presence characteristics and similarities with human isolates
Author(s) Koene, M.G.J.; Mevius, D.; Wagenaar, J.A.; Harmanus, C.; Hensgens, M.P.M.; Meetsma, A.M.; Putirulan, F.F.; Bergen, M.A.P. van; Kuijper, E.J.
Source Clinical Microbiology and Infection 18 (2012)8. - ISSN 1198-743X - p. 778 - 784.
DOI https://doi.org/10.1111/j.1469-0691.2011.03651.x
Department(s) CVI Bacteriology and Epidemiology
ID - Infectieziekten
CVI Infection Biology
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) tandem-repeat analysis - pcr ribotypes - disease - prevalence - infection - strains - calves - toxin - pigs
Abstract The presence and characteristics of Clostridium difficile were investigated in 839 faecal samples from seven different animal species in the Netherlands. The number of positive samples ranged from 3.4% (cattle) to 25.0% (dogs). Twenty-two different PCR ribotypes were identified. Among 96 isolates, 53% harboured toxin genes. All C. difficile isolates from pigs, cattle and poultry were toxinogenic, whereas the majority of isolates from pet animals consisted of non-toxinogenic PCR ribotypes 010 and 039. Ribotype 012 was most prevalent in cattle and ribotype 078 in pigs. No predominant ribotypes were present in horse and poultry samples. Overall, PCR ribotypes 012, 014 and 078 were the most frequently recovered toxinogenic ribotypes from animal samples. Comparison with human isolates from the Dutch Reference Laboratory for C. difficile at Leiden University Medical Centre (LUMC) showed that these types were also recovered from human hospitalized patients in 2009/2010, encompassing 0.8%, 11.4% and 9.8% of all isolates, respectively. Application of multiple-locus variable-number tandem-repeat analysis indicated a genotypic relation of animal and human ribotype 078 strains, but a clear genotypic distinction for ribotypes 012 and 014. We conclude that toxinogenic C. difficile PCR ribotypes found in animals correspond to PCR ribotypes associated with human disease in hospitalized patients in the Netherlands. Contrary to PCR ribotype 078, significant genetic differences were observed between animal and human PCR ribotype 012 and 014 isolates
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