Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 415314
Title The cps locus of Streptococcus suis serotype 16: Development of a serotype-specific PCR assay
Author(s) Wang, K.; Weixing, Fan; Wisselink, H.J.; Chengping, Lu
Source Veterinary Microbiology 153 (2011)3-4. - ISSN 0378-1135 - p. 403 - 406.
Department(s) CVI Infection Biology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) capsular types - pigs
Abstract Streptococcus suis serotype 16 can infect pigs and humans. We describe the identification and the characterization of the capsular polysaccharides synthesis locus of S. suis serotype 16. Using PCR primers flanking the capsular polysaccharides synthesis locus, a 30,101-bp fragment was amplified. Twenty-nine open reading frames related to transcriptional regulation, glycosyl transfer, oligosaccharide repeat unit polymerization, polysaccharide transport, sialic acid synthesis and modification were identified. The data suggests that the serotype 16 capsule is synthesized by a Wzy-dependent pathway. So far, no rapid and sensitive diagnostic method is available for detection of serotype 16 isolates. A serotype specific PCR test for the rapid and sensitive detection of S. suis serotype 16 was developed. Cross hybridization experiments of individual cps genes with chromosomal DNAs of 33 serotypes showed that the cps16G and cps16K genes hybridized with serotype 16 only. Primers based on cps16G were used to develop a serotype 16 specific PCR. The PCR assay was successfully used to identify S. suis serotype 16 in the 99 Chinese S. suis clinical isolates and 8 European isolates
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.