Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 415332
Title Live cell imaging reveals structural associations between the actin and microtubule cytoskeleton in Arabidopsis
Author(s) Sampathkumar, A.; Lindeboom, J.J.; Debolt, S.; Gutierrez, R.; Ehrhardt, D.W.; Ketelaar, T.; Persson, S.
Source The Plant Cell 23 (2011)6. - ISSN 1040-4651 - p. 2302 - 2313.
DOI https://doi.org/10.1105/tpc.111.087940
Department(s) Laboratory of Cell Biology
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) cortical microtubules - epidermal-cells - dinitroaniline herbicide - hair-cells - f-actin - organization - interacts - dynamics - array - microfilaments
Abstract In eukaryotic cells, the actin and microtubule (MT) cytoskeletal networks are dynamic structures that organize intracellular processes and facilitate their rapid reorganization. In plant cells, actin filaments (AFs) and MTs are essential for cell growth and morphogenesis. However, dynamic interactions between these two essential components in live cells have not been explored. Here, we use spinning-disc confocal microscopy to dissect interaction and cooperation between cortical AFs and MTs in Arabidopsis thaliana, utilizing fluorescent reporter constructs for both components. Quantitative analyses revealed altered AF dynamics associated with the positions and orientations of cortical MTs. Reorganization and reassembly of the AF array was dependent on the MTs following drug-induced depolymerization, whereby short AFs initially appeared colocalized with MTs, and displayed motility along MTs. We also observed that light-induced reorganization of MTs occurred in concert with changes in AF behavior. Our results indicate dynamic interaction between the cortical actin and MT cytoskeletons in interphase plant cells.
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