Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 415340
Title Comparative Transcriptome Analysis and Genome Assembly of Fusarium oxysporum f. sp. cubense
Author(s) Dita Rodriguez, M.A.; Herai, R.; Waalwijk, C.; Yamagishi, M.; Giachetto, P.; Ferreira, G.; Souza Jr., M.T.; Kema, G.H.J.
Source In: Program and abstracts of the ISHS/ProMusa symposium. Bananas and plantains:Toward sustainable global production and improved uses, Salvador, Bahia, Brazil, 10-14 October 2011. - Brazil : ISHS, ProMusa, Embrapa, Biodiversity International - p. 53 - 53.
Event Brazil : ISHS, ProMusa, Embrapa, Biodiversity International ISHS/ProMusa symposium. Bananas and plantains:Toward sustainable global production and improved uses, Salvador, Bahia, Brazil, 2011-10-10/2011-10-14
Department(s) PPO/PRI Biointeractions and Plant Health
PRI BIOINT Moleculair Phytopathology
Publication type Abstract in scientific journal or proceedings
Publication year 2011
Abstract Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt of banana, is a highly destructive and genetically diverse pathogen. Despite its economic importance, genomic information of Foc is poor and no transcriptomic analyses have been reported so far. By using 454 sequencing technology, we generated >2.5 million expressed sequenced tags (ESTs) from four Foc strains representing four vegetative compatibility groups (VCGs) and races that infect banana: race 1 (R1, VCG unknown but different from the others here described), race 2 (R2, VCG 0124), subtropical race 4 (SR4, VCG 0120) and tropical race (TR4, VCG 01213). The ESTs were obtained from libraries prepared from mRNA extracted from three physiological states (mycelia, conidia and germinated conidia), which were pooled at a 2:2:1 ratio. Most genes are represented in all libraries, but in silico comparative analyses identified a set of unique ESTs for each race (689 for R1, 974 for R2, 296 for SR4 and 555 for TR4), which constitute excellent candidates for diagnostics development, future plant-pathogen interaction studies and functional analyses. In subsequent analyses, a 40x sequencing-coverage (Illumina single reads) of TR4 genomic DNA was assembled in a de novo based methodology, resulting in a 29-kb N50 (463 contigs). Preliminary analyses show a high colinearity of EST and genomic data that significantly contributes to the quality of the assembly. Applications of these data will be further discussed
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