Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 415599
Title A generic (RT)-PCR test for caulimoviruses
Author(s) Vlugt, R.A.A. van der; Dullemans, A.M.
Source In: Book of Abstracts. The 3rd Conference of the International Working Group on Legume and Vegetable viruses (IWGLVV), Ljubljana, Slovenia, 20-23 August 2008. - Ljubljana, Slovenia : National Institute of Biology (NIB), Department of Biotechnology and Systems Biology - p. 70 (P18) - 70 (P18).
Event Ljubljana, Slovenia : National Institute of Biology (NIB), Department of Biotechnology and Systems Biology The 3rd Conference of the International Working Group on Legume and Vegetable viruses (IWGLVV), Ljubljana, 2008-08-20/2008-08-23
Department(s) PRI BIOINT Entomology & Virology
Publication type Abstract in scientific journal or proceedings
Publication year 2008
Abstract For a considerable number of viruses from different genera sequence data are available and for different virus genera general PCR primersets have been described. Not for all economically important viruses reliable detection methods are available yet. Caulimoviruses constitute a group of DNA plant viruses that cause diseases in a wide range of crops. To only a limited number of them specific diagnostics are available. Detection of poorly characterized or new caulimoviruses is often not possible or reliable. Based on available sequence data caulimovirus genus–specific primers were designed The primers were tested to available caulimoviruses from different crops. At least one generic primer set proofed capable of detection of all caulimovirus tested to date. Sequence analysis of the PCR products enabled subsequent identification of the individual viruses. Use of these primer sets will allow NPPOs and inspection services to more reliable monitor caulimovirus infections and their spread.
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