Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 416332
Title Growth of oil accumulating microalga Neochloris oleoabundans under alkaline-saline conditions
Author(s) Santos, A.M.; Janssen, M.G.J.; Lamers, P.P.; Evers, W.A.C.; Wijffels, R.H.
Source Bioresource Technology 104 (2012). - ISSN 0960-8524 - p. 593 - 599.
DOI https://doi.org/10.1016/j.biortech.2011.10.084
Department(s) Bioprocess Engineering
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) carbon-dioxide - flue-gas - biomass - photobioreactor - feedstocks - metabolism - economics - biodiesel - biofuels
Abstract The effect of elevated pH and salt concentration on the growth of the freshwater microalga Neochloris oleoabundans was investigated. A study was conducted in 24-well plates on the design of a growth medium and subsequently applied in a photobioreactor. An artificial seawater medium with reduced Ca(2+) and PO(4)(3-) could prevent mineral precipitation at high pH levels. Growth was characterized in this new medium at pH8.1 and at pH10.0, with 420mM of total salts. Specific growth rates of 0.08h(-1) at pH8.1 and 0.04h(-1) at pH10.0 were obtained under controlled turbidostat cultivation. The effect of nitrogen starvation on lipid accumulation was also investigated. Fatty acids content increased not only with nitrogen limitation but also with a pH increase (up to 35% in the dry biomass). Fluorescence microscopy gave visual proof that N. oleoabundans accumulates oil bodies when growing in saline conditions at high pH.
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