Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 416398
Title Regulator-based genome mining for novel bioactive compounds in soil bacteria
Author(s) Cheng, X.; Bruijn, I. de; Dekkers, E.; Voort, M. van der; Raaijmakers, J.M.
Source In: Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. - Wageningen, the Netherlands : - p. 26 - 26.
Event Wageningen, the Netherlands : EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 2011-11-01/2011-11-03
Department(s) Laboratory of Phytopathology
Publication type Abstract in scientific journal or proceedings
Publication year 2011
Abstract PO-06 Regulator-based genome mining for novel bioactive compounds in soil bacteria Xu Cheng, Irene de Bruijn, Ester Dekkers, Menno van der Voort and Jos M. Raaijmakers Laboratory of Phytopathology, Wageningen University, the Netherlands; Soil microorganisms represent an enormous resource for the discovery of novel genes and bioactive compounds, including antimicrobial metabolites and enzymes. Traditional methods to discover microbial products involve isolation and cultivation of a microorganism, followed by extraction, fractionation and structure elucidation of the bioactive compounds. The increase in the availability of microbial genomes has substantially changed our fundamental knowledge of the metabolic capabilities of microorganisms. Bioinformatics analyses of microbial genomes have revealed a large number of so-called orphan gene clusters, i.e. biosynthetic loci for which the corresponding pathways and metabolites are yet unknown. This study focuses on genome mining of Pseudomonas fluorescens strains for novel bioactive compounds and biosynthetic pathways. We adopted a targeted discovery strategy by generating mutations in two-component systems that act as global regulators of the production of secondary metabolites and exoenzymes in bacteria. A multidisciplinary approach involving activity bioassays, microarray-based expression profiling and genetic analyses resulted in the discovery of a new cluster of nonribosomal peptide synthetase genes that encode a putative novel compound with strong activities against fungal and oomycete pathogens.
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