Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 416405
Title Molecular handshakes: going deep into the identification of proteins interacting with the tomato NB-LRR NRC1
Author(s) Sueldo, D.; Prins, M.; Joosten, M.H.A.J.; Tameling, W.I.L.
Source In: Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. - Wageningen, the Netherlands : - p. 45 - 46.
Event Wageningen, the Netherlands : EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 2011-11-01/2011-11-03
Department(s) Laboratory of Phytopathology
Publication type Abstract in scientific journal or proceedings
Publication year 2011
Abstract PO-46 Molecular handshakes: going deep into the identification of proteins interacting with the tomato NB-LRR NRC1 Daniela SUELDO1, Marcel PRINS2, Matthieu H. A. J. JOOSTEN1 and Wladimir I.L. TAMELING1 1Wageningen UR, the Netherlands ; 2 Keygene, the Netherlands Plant disease resistance (R) proteins confer immunity to pathogens possessing the corresponding avirulence (Avr) proteins. Most R proteins localize intracellularly and only few (e.g. Cfs) are extracellular transmembrane proteins. Most intracellular R proteins belong to the nucleotide-binding and leucine-rich repeat (NB-LRR) class. Activation of NB-LRRs is often associated with induction of the hypersensitive response (HR), a form of programmed cell death. NRC1 (NB-LRR required for HR-associated cell death-1) is an NB-LRR from tomato, which participates in the signaling cascade required for Cf-mediated resistance to Cladosporium fulvum. Silencing of NRC1 in Nicotiana benthamiana results in an impaired HR induced by extracellular and intracellular R proteins. To understand how NRC1 regulates the defense response induced by multiple R proteins, we focus on the identification of interacting proteins. To this end, a yeast two-hybrid screening is currently in progress. A tomato cDNA library was constructed, derived from tomato ‘dying seedlings’ that undergo a synchronized HR; as they produce both Cf-4 as well as its cognate effector Avr4. Different domains of NRC1 are used as baits (NRC1-CC, NRC1-NB and NRC1-NB-ARC-LRR). In parallel, various tagged versions of NRC1 will be expressed in N. benthamiana for subsequent affinity-purification and identification of associated proteins by mass spectrometry. In most cases only a slight inhibition of NRC1 activity was observed. With this research we want to elucidate whether NRC1 is a true signaling hub for multiple R proteins, required to induce defense responses
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