Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 416408
Title ER-Quality Control is required for Cf-protein function
Author(s) Liebrand, T.W.H.; Cordewener, J.H.G.; America, A.H.P.; Joosten, M.H.A.J.
Source In: Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. - Wageningen, The Netherlands : - p. 34 - 34.
Event Wageningen, The Netherlands : EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 2011-11-01/2011-11-03
Department(s) Laboratory of Phytopathology
PRI BIOS Applied Genomics & Proteomics
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2011
Abstract PO-22 ER-Quality Control is required for Cf-protein function Thomas Liebrand1, Jan Cordewener2, Antoine America2, and Matthieu Joosten1 1 Laboratory of Phytopathology, Wageningen University, The Netherlands 2 Plant Research International, Business Unit Bioscience, Wageningen University and Research Centre, The Netherlands The plasma membrane-resident tomato (Solanum lycopersicum) Cf receptor-like proteins recognize Avr effectors of the fungal pathogen Cladosporium fulvum. To date, few host components involved in Cf-biogenesis and signaling have been identified. We perform affinity purification from plant extracts to identify Cf-interacting proteins. We transiently expressed a Cf-4-eGFP fusion protein in Nicotiana benthamiana by agroinfiltration. Immunoprecipitation of Cf-4-eGFP and subsequent mass spectrometry analysis revealed the endoplasmic reticulum (ER)-resident HSP70 chaperone Binding Protein 1 BiP1 as co-purifying protein. Co-Immunoprecipitation assays revealed that BiP also interacts with transiently expressed eGFP tagged Cf-2, Cf-9, Cf-4E and Peru2 (a Cf homolog). BiP is part of an ER-chaperone complex together with ERdj3b and SDF2. Besides the BiP chaperone complex, the N-linked glycosylation pathway including the ER-localized lectin-like chaperone calreticulins (CRTs) are involved in ER-quality control (ER-QC). Using Virus-Induced Gene Silencing in transgenic N. benthamiana:Cf-4 plants we studied the role of ER-QC for Cf-4 functioning. We discovered that silencing of specifically CRT3-likes reduced the Avr4-triggred HR, but not the HR triggered by the CC-NB-LRR autoactive mutant Rx(D460V) or the apoptotic factor BAX. Silencing of NbBiP1 did not reduce Avr4-triggered HR, which may be explained by redundancy with one of the other two BiPs. Acknowledgements This work is supported by CBSG2012 PG6 and CBSG Hotel project TD8-08.
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