Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 416409
Title Transcriptome analysis on Verticillium-infected tomato to identify genes involved in host defense
Author(s) Yanes Paz, E.; Rehman, S.; Bai, Y.; Santos Bermudez, R.; Borrás-Hidalgo, O.; Thomma, B.P.H.J.
Source In: Book of Abstracts of the EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 1-3 November 2011. - Wageningen, The Netherlands : - p. 48 - 49.
Event Wageningen, The Netherlands : EPS PhD Autumn School 'Host-Microbe Interactomics', Wageningen, The Netherlands, 2011-11-01/2011-11-03
Department(s) Laboratory of Phytopathology
Laboratory of Plant Breeding
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2011
Abstract PO-52 Transcriptome analysis on Verticillium-infected tomato to identify genes involved in host defense. Ermis Yanes-Paz1,2*, Sajid Rehman1, Yuling BAI1, Ramón Santos Bermudez2, Orlando Borrás-Hidalgo3, Bart P.H.J. Thomma1 1Laboratory of Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands. 2Bioplants Center, Ciego de Avila, Cuba. 3Center for Genetic Engineering and Biotechnology, Havana, Cuba * eyanes@bioplantas.cu Despite the economical importance of Verticillium spp., relatively little is known about the molecular basis of Verticillium pathogenicity and host resistance against this fungus. In the current work comparative transcriptomics was performed on susceptible and resistant tomato lines of the cultivar MoneyMaker inoculated with a race 1 isolate of Verticillium using cDNA-AFLP in an attempt to identify key components involved in Ve1 mediated resistance. As a resistant line, Ve1 transgenic MoneyMaker was used (Fradin et al., 2009). A total number of 176 selective primer combinations were tested and 1434 differentially expressed transcript-derived fragments (DE-TDFs) were identified. As the DE-TDFs that are up-regulated specifically in resistant tomato upon Verticillium inoculation (302 DE-TDFs) may be involved in host resistance, these are functionally analyzed using virus-induced gene silencing (VIGS). It was shown that silencing 54 out of 192 TDFs reproducible compromised Ve1 mediated Verticillium resistance. Further study on five selected candidates have shown that the silencing of three of them leads to an increased of fungus colonization.
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