Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 416801
Title Enzymatic Production and Characterization of Konjac Glucomannan Oligosaccharides
Author(s) Albrecht, S.A.; Muiswinkel, G.C.J. van; Xu, Jiqiang; Schols, H.A.; Voragen, A.G.J.; Gruppen, H.
Source Journal of Agricultural and Food Chemistry 59 (2011)23. - ISSN 0021-8561 - p. 12658 - 12666.
DOI http://dx.doi.org/10.1021/jf203091h
Department(s) Food Chemistry Group
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) in-vitro fermentation - human colonic microbiota - dietary modulation - prebiotics - purification - microflora - mannan - inulin - pigs
Abstract Enzymes from a balanced human gut flora are promising tools to design prebiotic oligosaccharides. In this study, we investigated the action of enzymes from fecal bacteria on the complex polysaccharide konjac glucomannan (KGM). The oligosaccharides produced were compared to oligosaccharides from KGM digests with fungal endo-ß-(1,4)-glucanase (EG) or endo-ß-(1,4)-mannanase (EM). For this purpose, the oligosaccharides from the different digests were first studied for their structural characteristics like monosugar composition and exo-enzymatic degradability, as monitored by capillary electrophoresis with laser-induced fluorescence detection. Whereas the oligosaccharides produced by EG and EM were characteristic for the selectivity of the respective enzyme in cleaving the mannose-/glucose-sugar linkages of KGM, oligosaccharides produced by the fecal enzymes did not point to a sugar-selective degradation. The oligosaccharide fragments from the different digests indicated the KGM polysaccharide to be composed of a backbone composed of short mannose and glucose sequences, to which branches rich in mannose are attached.
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