Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 417261
Title Quantitative trace analysis of eight chloramphenicol isomers in urine by chiral liquid chromatography coupled to tandem mass spectrometry
Author(s) Berendsen, B.J.A.; Essers, M.L.; Stolker, A.A.M.; Nielen, M.W.F.
Source Journal of Chromatography. A, Including electrophoresis and other separation methods 1218 (2011)41. - ISSN 0021-9673 - p. 7331 - 7340.
DOI http://dx.doi.org/10.1016/j.chroma.2011.08.046
Department(s) RIKILT - R&C Diergeneesmiddelen
RIKILT - R&C Groeibevorderaars
Laboratory for Organic Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) human plasma - antibiotic chloramphenicol - enantiomeric separation - residues - meat - dextramycin - 2002/657/ec - validation - seafood - drugs
Abstract Chloramphenicol is a broad-spectrum antibiotic with, apart from its human medicinal use, veterinary abuse in all major food-producing animals. Chloramphenicol occurs in four stereoisomers (all para-nitro substituted) and furthermore four meta-nitro analogs of chloramphenicol exist. In this paper these are referred to as eight chloramphenicol isomers. According to EU regulations an analytical method should be able to discriminate the analyte from interfering substances that might be present in the sample, including isomers. For the first time a quantitative method for the analysis of trace levels of eight chloramphenicol isomers in urine by chiral liquid chromatography in combination with tandem mass spectrometric detection is reported. The separation of the isomers on the analytical column, the clean-up of urine and the selectivity of the monitored product ions turned out to be critical parameters. To obtain reproducible retention isocratic elution on a chiral AGP column was applied. For urine samples matrix compounds present in the final extract caused decreased retention of the isomers on the chiral stationary phase and a lack of chromatographic resolution. Therefore an extended clean-up procedure that combines solid phase extraction and liquid–liquid extraction had to be developed. The final method was fully validated and showed satisfactory performance for all isomers with decision limits (CCa) ranging from 0.005 to 0.03 µg L-1 and within-laboratory reproducibility of all isomers below 20% at the minimum required performance limit level of 0.3 µg L-1. --------------------------------------------------------------------------------
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