Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 417273
Title Tailored Microarray Platform for the Detection of Marine Toxins
Author(s) Bovee, T.F.H.; Hendriksen, P.J.M.; Portier, L.; Wang, S.; Elliott, C.T.; Egmond, H.P. van; Nielen, M.W.F.; Peijnenburg, A.A.C.M.; Hoogenboom, L.A.P.
Source Environmental Science and Technology 45 (2011)20. - ISSN 0013-936X - p. 8965 - 8973.
DOI http://dx.doi.org/10.1021/es2011385
Department(s) Rikilt B&T Toxicologie en Effectanalyse
RIKILT - R&C Natuurlijke Toxinen en Pesticiden
RIKILT - R&C Groeibevorderaars
Laboratory for Organic Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) harmful algal blooms - human health - shellfish toxins - microplate assay - climate-change - okadaic acid - bioassays - cyanobacteria - yessotoxins - polyether
Abstract Currently, there are no fast in vitro broad spectrum screening bioassays for the detection of marine toxins. The aim of this study was to develop such an assay. In gene expression profiling experiments 17 marker genes were provisionally selected that were differentially regulated in human intestinal Caco-2 cells upon exposure to the lipophilic shellfish poisons azaspiracid-1 (AZA1) or dinophysis toxin-1 (DTX1). These 17 genes together with two control genes were the basis for the design of a tailored microarray platform for the detection of these marine toxins and potentially others. Five out of the 17 selected marker genes on this dedicated DNA microarray gave clear signals, whereby the resulting fingerprints could be used to detect these toxins. CEACAM1, DDIT4, and TUBB3 were up-regulated by both AZA1 and DTX1, TRIB3 was up-regulated by AZA1 only, and OSR2 by DTX1 only. Analysis by singleplex qRT-PCR revealed the up- and down-regulation of the selected RGS16 and NPPB marker genes by DTX1, that were not envisioned by the new developed dedicated array. The qRT-PCR targeting the DDIT4, RSG16 and NPPB genes thus already resulted in a specific pattern for AZA1 and DTX1 indicating that for this specific case qRT-PCR might a be more suitable approach than a dedicated array
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