Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 417316
Title BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle
Author(s) Hoffmann, C.; Eiden, M.; Kaatz, M.; Keller, M.; Ziegler, U.; Rogers, R.; Hills, B.; Balkema-Buschmann, A.; Keulen, L. Van; Jacobs, J.G.; Groschup, M.H.
Source Veterinary Research 42 (2011). - ISSN 0928-4249 - 12 p.
Department(s) CVI Infection Biology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) bovine spongiform encephalopathy - follicular dendritic cells - enteric nervous-system - prion protein - tse pathogenesis - sheep - scrapie - disease - prpsc - spread
Abstract To establish bovine spongiform encephalopathy (BSE) public health protection measures it is important to precisely define the cattle tissues considered as specified risk materials (SRM). To date, in pre-clinical BSE infected cattle, no evidence of the BSE agent had been found in the gut outside of the ileal Peyer's Patches. This study was undertaken to determine when and where the pathological prion protein (PrPSc) and/or BSE infectivity can be found in the small intestine of cattle 4 to 6 months of age, orally challenged with BSE. Samples of the jejunum, the ileum and the ileocaecal junction from 46 BSE infected cattle, culled from 1 up to 44 months post infection (mpi) were examined by immunohistochemistry. Samples from cattle 8 mpi to 20 mpi were additionally studied by PTA Western blot, rapid tests, and by mouse (TgbovXV) bioassay. In doing so nearly all of the cattle, from 4 up to 44 mpi, had detectable amounts of PrPSc and/or infectivity in the distal ileum. In the distal ileum clear time-dependent variations were visible concerning the amount of PrPSc, the tissue structures affected, and the cells involved. BSE infectivity was found not only in the ileum and ileocaecal junction but also in the jejunum. The systematic approach of this study provides new data for qualitative and quantitative risk assessments and allows defining bovine SRM more precisely.
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