Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 417449
Title Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control.
Author(s) Hoffmann, B.; Blome, S.; Bonulauri, P.; Fernández-Pinero, J.; Greiser-Wilke, I.; Haegeman, A.; Isaksson, M.; Koenen, F.; Leblanc, N.; Leifer, I.; Potier, M.F. Le; Loeffen, W.; Rasmussen, T.B.; Stadejek, T.; Stahl, K.; Tignon, M.; Uttenthal, A.; Poel, W.H.M. van der
Source Journal of Veterinary Diagnostic Investigation 23 (2011)5. - ISSN 1040-6387 - p. 999 - 1004.
DOI http://dx.doi.org/10.1177/1040638711416849
Department(s) CVI Virology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) rt-pcr assay - pestiviruses - validation - infection - virulence - vaccine - taqman - probes - signs - pigs
Abstract The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house real-time RT-PCR protocols and a standardized protocol commonly used by the Friedrich-Loeffler-Institute (FLI) on a panel of well-characterized samples. In general, all participants produced results within the acceptable range. The FLI assay, several in-house assays, and the commercial kits had high analytical sensitivity and specificity values. Nevertheless, some in-house systems had unspecific reactions or suboptimal sensitivity with only a single CSFV genotype. Follow-up actions involved either improvement of suboptimal assays or replacement of specific laboratory assays with the FLI protocol, with or without modifications. In conclusion, the ring trial showed reliability of classical swine fever diagnosis on an international level and helped to optimize CSFV-specific RT-PCR diagnostics.
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