Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 417469
Title Ultrasonic or accelerated solvent extration followed by U-HPLC-high mass accuracy MS for screening of pharmaceuticals and fungicides in soil and plant samples
Author(s) Chitescu, C.L.; Oosterink, J.E.; Jong, J. de; Stolker, A.A.M.
Source Talanta 88 (2012). - ISSN 0039-9140 - p. 653 - 662.
DOI http://dx.doi.org/10.1016/j.talanta.2011.11.054
Department(s) RIKILT - R&C Diergeneesmiddelen
RIKILT - R&C Natuurlijke Toxinen en Pesticiden
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) pressurized-liquid extraction - veterinary antibiotics - chromatography - spectrometry - environment - fate - food
Abstract Different veterinary pharmaceuticals are used in agricultural livestock becoming a source of environment contamination. Furthermore, no regulation exists for the concentration limits of pharmaceuticals in soil or water. Monitoring programs for environment contamination with pharmaceuticals are needed, requiring new sensitive and selective screening methods. The present study focuses on developing a method for the simultaneous scanning of forty-two compounds (pharmaceuticals, azole biocides and fungicides) in soil and plant material samples. For extraction purposes the use of ultrasonic assisted and accelerated solvent extraction (ASE) were compared. The extract was purified and concentrated by applying a solid phase extraction step followed by ultra-high-performance-chromatographic separation and accurate-mass spectrometric detection using Exacte Orbitrap technology (FWHM 50,000). The effects of the different extraction solvents and conditions on the extraction efficiency were tested. Although both extraction approaches are applicable the optimal extraction efficiency was obtained by applying accelerated solvent extraction using solvent mixtures containing acetone for soil and methanol for plant samples. An ASE process has been validated for the determination of selected pharmaceuticals and fungicides in soil and in plant material. The recoveries from soil samples were >70% for more than 68% of the compounds. The levels of detection were =10 µg kg-1 for 93% of the compounds tested. The recoveries from plant material were >70% for 64% of the compounds tested. The levels of detection were =10 µg kg-1 for 66% of the compounds. The developed method was used to screen soil and plant material collected throughout the Netherlands and oxytetracycline residues were detected. --------------------------------------------------------------------------------
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