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Record number 417805
Title Current methods of analysis for the determination of trichothecene mycotoxins in food
Author(s) Meneely, J.P.; Ricci, F.; Egmond, H.P. van; Elliott, C.T.
Source TrAC : Trends in Analytical Chemistry 30 (2011)2. - ISSN 0165-9936 - p. 192 - 203.
Department(s) RIKILT - R&C Natuurlijke Toxinen en Pesticiden
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) performance liquid-chromatography - differential-pulse polarography - tandem mass-spectrometry - monoclonal-antibodies - electrochemical immunosensor - deoxynivalenol don - b trichothecenes - aflatoxin b-1 - wheat - immunoassay
Abstract This article describes the trends in analytical techniques for the determination of trichothecene mycotoxins, namely deoxynivalenol, and T-2 and HT-2 toxins in cereals and cereal products with particular emphasis on screening and rapid approaches. The driving force behind the changing methodologies is mainly attributed to legislative demands. However, for commercial and governmental testing laboratories, the need to use validated official methods is ever increasing to ensure quality assurance of results. Much research has been undertaken to improve screening assays, highlighted by the number of new methods using a variety of formats and platforms, including optical and electrochemical biosensors. Significant advances in the traditional reference methods have also been demonstrated in addition to the emergence of a variety of commercial immunoaffinity and solid-phase extraction columns for clean up. The use of liquid chromatography coupled to tandem mass spectrometry for mycotoxin detection is ever increasing, allowing simultaneous determination of many toxins in various sample matrices.
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