Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 417956
Title Extremely low frequency electromagnetic field exposure does not modulate Toll-like receptor signaling in human peripheral blood mononuclear cells
Author(s) Kleijn, S. de; Bouwens, M.; Verburg-van Kemenade, B.M.L.; Cuppen, J.J.M.; Ferwerda, G.; Hermans, P.
Source Cytokine 54 (2011)1. - ISSN 1043-4666 - p. 43 - 50.
Department(s) Cell Biology and Immunology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) magnetic-fields - cytokine production - childhood leukemia - interferon-gamma - pooled analysis - stimulation - macrophages - induction - tlr4 - rats
Abstract The effects of extremely low frequency electromagnetic fields (ELF-EMF) on human health remain unclear. It has been reported that ELF-EMF may modulate the innate immune response to microorganisms in animal models and mammalian cell-lines. With the recently gained insight in innate immune signaling and the discovery of pattern recognition, we aim to study whether ELF-EMF modulates innate inflammatory signaling pathways. We used human peripheral blood mononuclear cells (PBMCs), isolated from blood from healthy volunteers, which we stimulated with specific TLR2 and TLR4 ligands, and with several microorganisms. The cells were subsequently exposed in Bdc = 3 lT to a highly controlled and standardized ELF-EMF signal (20–5000 Hz, Bac = 5 lT, 30 min) and cytokine production was measured at different time points after stimulation. No significant difference in immune response, as reflected by IL-1b, IL-6, TNFa, IL-8 and IL-10 production, could be detected after stimulation with LPS (TLR4 ligand), Pam3Cys (TLR2 ligand) or a panel of heat killed microorganisms: Mycobacterium tuberculosis, Salmonella typhimurium, Candida albicans, Aspergillus fumigatus and Staphylococcus aureus (multiple TLR ligands). We therefore conclude that under our experimental conditions, ELF-EMF does not modulate the innate immune response of human primary cells after TLR stimulation in vitro.
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