Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 417997
Title Quantitative trace analysis of a broad range of antiviral drugs in poultry muscle using column-switch liquid chromatography coupled to tandem mass spectrometry
Author(s) Berendsen, B.J.A.; Wegh, R.S.; Essers, M.L.; Stolker, A.A.M.; Weigel, S.
Source Analytical and Bioanalytical Chemistry 402 (2012)4. - ISSN 1618-2642 - p. 1611 - 1623.
DOI http://dx.doi.org/10.1007/s00216-011-5581-3
Department(s) RIKILT - R&C Diergeneesmiddelen
RIKILT - R&C Groeibevorderaars
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) metabolite oseltamivir carboxylate - solid-phase extraction - influenza-a virus - lc-ms/ms method - human plasma - rat plasma - human serum - resistance - amantadine - ribavirin
Abstract A liquid chromatography–tandem mass spectrometry method for the analysis of seven antiviral drugs, zanamivir, ribavirin, oseltamivir, oseltamivir carboxylate, amantadine, rimantadine and arbidol, in poultry muscle is reported. The antiviral drugs were extracted from the homogenized poultry muscle sample using methanol. The extract was purified using tandem solid-phase extraction combining a cation exchange cartridge and a phenylboronic acid cartridge. To prevent excessive matrix effects, the analytes were separated from the matrix constituents using a column-switch liquid chromatography system combining a reversed-phase and a Hypercarb analytical column. Detection was carried out using tandem mass spectrometry. The method was fully validated according to 2002/657/EC [1] and proved to be adequate for quantification and confirmation of zanamivir and ribavirin at 10 µg kg-1, oseltamivir, oseltamivir carboxylate, amantadine and rimantadine at levels below 1.0 µg kg-1 and for qualitative confirmatory analysis of arbidol at levels below 1 µg kg-1.
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