Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 418038
Title Filter-aided sample preparation with dimethyl labeling to identify and quantify milk fat globule membrane proteins.
Author(s) Lu, J.; Boeren, J.A.; Vries, S.C. de; Valenberg, H.J.F. van; Vervoort, J.J.M.; Hettinga, K.A.
Source Journal of Proteomics 75 (2011)1. - ISSN 1874-3919 - p. 34 - 43.
DOI http://dx.doi.org/10.1016/j.jprot.2011.07.031
Department(s) Product Design and Quality Management Group
Biochemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) quantitative proteomics - xanthine-oxidoreductase - mammary-gland - secretion - identification - expression - origin
Abstract Bovine milk is a major nutrient source in many countries and it is produced at an industrial scale. Milk is a complex mixture of proteins, fats, carbohydrates, vitamins and minerals. The composition of the bovine milk samples can vary depending on the genetic makeup of the bovine species as well as environmental factors. It is therefore important to study the qualitative and quantitative differences of bovine milk samples. Proteins in milk can be present in casein micelles, in the serum (the water soluble fraction) or in fat globules. These fat globules have a double membrane layer with proteins being bound to or being incapsulated in the membrane layer. The identification and molecular composition of the milk proteins have gained increased interest in recent years. Proteomic techniques make it now possible to identify up to many thousands of proteins in one sample, however quantification of proteins is as yet not straightforward. We analyzed the proteins of the milk fat globule membrane using dimethyl labeling methods combined with a filter-aided sample preparation protocol. Using these methods, it is now possible to quantitatively study the detailed protein composition of many milk samples in a short period of time.
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