Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 418058
Title The effect of low oxygen with and without steady-state hydrogen peroxide on cytokine gene and protein expression of monocyte-derived macrophages - biomed 2011
Author(s) Owegi, H.; Bouwens, M.; Egot-Lemaire, S.; Mueller, S.; Geib, R.W.; Waite, G.N.
Source Biomedical Sciences Instrumentation 47 (2011). - ISSN 0067-8856 - p. 58 - 63.
Department(s) Cell Biology and Immunology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2011
Abstract An early event during inflammation and infection is the migration of monocytes into tissues where they differentiate into macrophages. Such monocyte-derived macrophages face an unfavorable environment characterized by extremely low oxygen tension and accumulation of reactive oxygen species such as hydrogen peroxide. Previous experiments showed that a macrophage cell line cultured under these conditions responded to inflammatory stimulants with an increased respiratory burst compared to cells kept at regular ambient oxygen conditions. The objective of the present study was to test if this effect was accompanied by an increased cytokine production. Using cytokine protein arrays and real time gene expression analysis, we indeed found that low oxygen exposure increased expression of characteristic macrophage inflammatory cytokines such as IL-1, IL-6, and TNF-alpha. Interestingly, cells exposed to low oxygen and steady-state hydrogen peroxide levels did not show this increase, indicating different cell mechanisms might be involved in increasing macrophage hydrogen peroxide and cytokine release.
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