Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 418331
Title Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules
Author(s) Rajan, B.; Fernandes, J.M.O.; Caipang, C.M.A.; Kiron, V.; Rombout, J.H.W.M.; Brinchmann, M.
Source Fish and Shellfish Immunology 31 (2011)2. - ISSN 1050-4648 - p. 224 - 231.
Department(s) Cell Biology and Immunology
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) channel catfish - epidermal mucus - lepeophtheirus-salmonis - ictalurus-punctatus - neutrophil elastase - intestinal mucus - conger-myriaster - cyprinus-carpio - binding-lectin - cyclophilin-a
Abstract The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR
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