Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 418733
Title Influence of aerobic and anaerobic conditions on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in Luria-Bertani broth, farm-yard manure and slurry
Author(s) Semenov, A.V.; Overbeek, L.S. van; Termorshuizen, A.J.; Bruggen, A.H.C. van
Source Journal of Environmental Management 92 (2011)3. - ISSN 0301-4797 - p. 780 - 787.
DOI http://dx.doi.org/10.1016/j.jenvman.2010.10.031
Department(s) PPO/PRI Biointeractions and Plant Health
PRI BIOINT Ecological Interactions
Biological Farming Systems
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) gradient gel-electrophoresis - amended soil - cow manure - united-states - aggregative behavior - o157-h7 survival - fresh produce - ribosomal-rna - dairy manure - swine manure
Abstract The influence of aerobic and anaerobic conditions on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium was investigated in microcosms with broth, cattle manure or slurry. These substrates were inoculated with a green fluorescent protein transformed strain of the enteropathogens at 107 cells g-1 dry weight. Survival data was fitted to the Weibull model. The survival curves in aerobic conditions generally showed a concave curvature, while the curvature was convex in anaerobic conditions. The estimated survival times showed that E. coli O157:H7 survived significantly longer under anaerobic than under aerobic conditions. Survival ranged from approximately. 2 weeks for aerobic manure and slurry to more than six months for anaerobic manure at 16 °C. On average, in 56.3% of the samplings, the number of recovered E. coli O157:H7 cells by anaerobic incubation of Petri plates was significantly (p <0.05) higher in comparison with aerobic incubation. Survival of Salmonella serovar Typhimurium was not different between aerobic and anaerobic storage of LB broth or manure as well as between aerobic and anaerobic incubation of Petri dishes. The importance of changes in microbial community and chemical composition of manure and slurry was distinguished for the survival of E. coli O157:H7 in different oxygen conditions.
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