Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 419384
Title Immobilization to prevent enzyme incompatibility with proteases
Author(s) Vossenberg, P.; Beeftink, H.H.; Cohen Stuart, M.A.; Tramper, J.
Source Biocatalysis and Biotransformation 29 (2011)6. - ISSN 1024-2422 - p. 288 - 298.
Department(s) Bioprocess Engineering
Physical Chemistry and Colloid Science
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) candida-antarctica - peptide-synthesis - organic-synthesis - crystal-structure - water activity - lipase-b - deactivation - improvement - autolysis - proteins
Abstract Enzyme incompatibility is a problem in multi-enzyme processes that involve a non-specific protease, such as Alcalase. An example is the one-pot enzymatic synthesis of peptides catalyzed by a lipase and a protease. The incompatibility between lipase B from Candida antarctica (CalB) and Alcalase was studied. To what extent immobilization of both or either CalB or Alcalase onto macroporous beads helps to prevent hydrolysis of CalB by Alcalase was evaluated. The rate of activity loss of native and immobilized CalB in the absence and presence of native and immobilized Alcalase was calculated from the rate of triacetin hydrolysis. Immobilization of both or either CalB or Alcalase onto macroporous beads was found to be effective in largely preventing hydrolysis of CalB by Alcalase
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