Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 419543
Title A high intake of industrial or ruminant trans fatty acids does not affect the plasma proteome in healthy men
Author(s) Roos, B. de; Wanders, A.J.; Wood, S.; Horgan, G.; Rucklige, G.; Reid, M.; Siebelink, E.; Brouwer, I.A.
Source Proteomics 11 (2011)19. - ISSN 1615-9853 - p. 3928 - 3934.
DOI https://doi.org/10.1002/pmic.201100163
Department(s) Chair Nutrition and Health over the Lifecourse
Chair Nutrition and Disease
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) conjugated linoleic-acid - lipid-peroxidation - insulin-resistance - biomarker discovery - risk-factors - cholesterol - disease - serum - supplementation - inflammation
Abstract Consumption of industrial trans fat raises the risk of cardiovascular disease, but it is unclear whether cis9,trans11-conjugated linoleic acid (CLA) – a trans fatty acid in dairy products – modulates disease development. We investigated the effects of complete diets providing 7% of energy as industrial trans fat or cis9, trans11 CLA, compared with oleic acid, on regulation of plasma proteins in 12 healthy men. Diets were provided for 3¿wk each, in random order. Plasma was collected at the end of each 3 wk intervention period, depleted of its 12 most abundant proteins and analyzed by 2-DE. Principal component analysis of protein spot intensity values revealed that the nature of the dietary intervention did not significantly affect the plasma proteome. The intervention provided in the 1st period produced a significant treatment effect compared with the interventions provided in the other two periods, and there was a significant subject effect. In conclusion, the nature of an extreme dietary intervention, i.e. 7% of energy provided by industrial trans fat or cis9,trans11 CLA, did not markedly affect the plasma proteome. Thus plasma proteomics using 2-DE appears, by and large, an unsuitable approach to detect regulation of plasma proteins due to changes in the diet
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