Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 420078
Title Marginal selenium deficiency down-regulates inflammation-related genes in splenic leukocytes of the mouse
Author(s) Kipp, A.P.; Banning, A.; Schothorst, E.M. van; Meplan, C.; Coort, S.L.; Evelo, C.; Keijer, J.; Hesketh, J.; Brigelius, R.
Source Journal of Nutritional Biochemistry 23 (2012)9. - ISSN 0955-2863 - p. 1170 - 1177.
DOI https://doi.org/10.1016/j.jnutbio.2011.06.011
Department(s) Human and Animal Physiology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) kappa-b activation - smooth-muscle-cells - response syndrome - immune-response - glutathione-peroxidase - protein-biosynthesis - t-cell - expression - selenoproteins - macrophages
Abstract Moderate selenium deficiency may lead to an impaired capacity to cope with health challenges. Functional effects of suboptimal selenium intake are not fully known, and biomarkers for an insufficient selenium supply are inadequate. We therefore fed mice diets of moderately deficient or adequate selenium intake for 6 weeks. Changes in global gene expression were monitored by microarray analysis in splenic leukocytes. Genes for four selenoproteins, Sepw1, Gpx1, Selh and Sep15, were the most significantly down-regulated in moderate selenium deficiency, and this was confirmed by quantitative polymerase chain reaction (qPCR). Classification of significantly affected genes revealed that processes related to inflammation, heme biosynthesis, DNA replication and transcription, cell cycle and transport were affected by selenium restriction. Down-regulation by moderate selenium deficiency of specific genes involved in inflammation and heme biosynthesis was confirmed by qPCR. Myeloperoxidase and lysozyme activities were decreased in selenium-restricted leukocytes, providing evidence for functional consequences. Genes for 31 nuclear factor (NF)-¿B targets were down-regulated in moderate selenium deficiency, indicating an impaired NF-¿B signaling. Together, the observed changes point to a disturbance in inflammatory response. The selenoproteins found here to be sensitive to selenium intake in murine leukocytes might also be useful as biomarkers for a moderate selenium deficiency in humans.
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