Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 420080
Title Alterations in hepatic one-carbon metabolism and related pathways following a high-fat dietary intervention
Author(s) Rubio-Aliaga, I.; Roos, B.; Sailer, M.; McLoughlin, G.; Schothorst, E.M. van; Erk, M.J. van; Keijer, J.; Müller, M.R.; Boekschoten, M.V.; Bachmair, E.M.; Coort, S.L.; Evelo, C.; Gibney, M.J.; Daniel, H.; Muller, M.; Kleemann, R.; Brennan, L.
Source Physiological genomics 43 (2011)8. - ISSN 1094-8341 - p. 408 - 416.
Department(s) Human and Animal Physiology
Chair Nutrition Metabolism and Genomics
Publication type Refereed Article in a scientific journal
Publication year 2011
Keyword(s) homocysteine s-methyltransferase - induced insulin-resistance - oxidative stress - deficient mice - ikk-beta - plasma - choline - liver - disease - obesity
Abstract Obesity frequently leads to insulin resistance and the development of hepatic steatosis. To characterize the molecular changes that promote hepatic steatosis, transcriptomics, proteomics, and metabolomics technologies were applied to liver samples from C57BL/6J mice obtained from two independent intervention trials. After 12 wk of high-fat feeding the animals became obese, hyperglycemic, and insulin resistant, had elevated levels of blood cholesterol and VLDL, and developed hepatic steatosis. Nutrigenomic analysis revealed alterations of key metabolites and enzyme transcript levels of hepatic one-carbon metabolism and related pathways. The hepatic oxidative capacity and the lipid milieu were significantly altered, which may play a key role in the development of insulin resistance. Additionally, high choline levels were observed after the high-fat diet. Previous studies have linked choline levels with insulin resistance and hepatic steatosis in conjunction with changes of certain metabolites and enzyme levels of one-carbon metabolism. The present results suggest that the coupling of high levels of choline and low levels of methionine plays an important role in the development of insulin resistance and liver steatosis. In conclusion, the complexities of the alterations induced by high-fat feeding are multifactorial, indicating that the interplay between several metabolic pathways is responsible for the pathological consequences.
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