Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 420081
Title Interference of flavonoids with enzymatic assays for the determination of free fatty acid and triglyceride levels
Author(s) Hoek-van den Hil, E.F.; Beekmann, K.; Keijer, J.; Hollman, P.C.H.; Rietjens, I.; Schothorst, E.M. van
Source Analytical and Bioanalytical Chemistry 402 (2012)3. - ISSN 1618-2642 - p. 1389 - 1392.
Department(s) Sub-department of Toxicology
Human and Animal Physiology
Rikilt B&T Toxicologie en Effectanalyse
Publication type Refereed Article in a scientific journal
Publication year 2012
Keyword(s) dietary flavonoids - thyroid peroxidase - quercetin - metabolites - rats - myeloperoxidase - polyphenols - inhibition - oxidation - cells
Abstract Flavonoids are bioactive food compounds with potential lipid-lowering effects. Commercially available enzymatic assays are widely used to determine free fatty acid (FFA) and triglyceride (TG) levels both in vivo in plasma or serum and in vitro in cell culture medium or cell lysate. However, we have observed that various flavonoids interfere with peroxidases used in these enzymatic assays, resulting in incorrect lower FFA and TG levels than actually present. Furthermore, addition of isorhamnetin or the major metabolite of the flavonoid quercetin in human and rat plasma, quercetin-3-O-glucuronide, to murine serum also resulted in a significant reduction of the detected TG levels, while a trend was seen for FFA levels. It is concluded that when applying these assays, vigilance is needed and alternative analytical methods, directly assessing FFA or TG levels, should be used for studying the biological effects of flavonoids on FFA and TG levels.
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